High-throughput Wastewater SARS-CoV-2 Detection Pipeline
By: Caroline H Sheikhzadeh, Luis Gonzalez-barranca, Anna M Adams, Olivia Ott, Smruthi Karthikeyan, Lisa Marotz, Greg Humphrey
Streamlined, cost efficient process for sample collection, magnetic-bead based concentration, nucleic acid extraction, and multiplexed PCR for wastewater based detection of SARS-CoV-2 viral particles in up to 96 samples per run.
SARS-CoV-2 sequencing using PrimalSeq-LibAmp for ONT
By: Ezra Kurzban, Sarah Perkins, Catie Anderson, Madison Schwab, Mark Zeller
Protocol for sequencing SARS-CoV-2 using a long amplicon scheme for PrimalSeq. The average length of amplicons in this protocol is ~1200 nt, making this well-suited to sequencing on Oxford Nanopore platforms.
SARS-CoV-2 sequencing using ARTIC PrimalSeq-LibAmp
By: Catie Anderson, Mark Zeller
Updated protocol for sequencing SARS-CoV-2 using a shorter amplicon scheme for PrimalSeq. The average length of amplicons in this protocol is ~250 nt, making this well-suited to sequencing on NovaSeq, iSeq, and NextSeq platforms.
SARS-CoV-2 sequencing using ARTIC PrimalSeq-Nextera
By: Catie Anderson, Mark Zeller
Protocol for sequencing SARS-CoV-2 using ARTIC PrimalSeq. The average length of amplicons in this protocol is ~400 nt, making this well-suited to sequencing on the MiSeq.
3D printed nasopharyngeal swabs with wrapped rayon fibers
Justin Ryan, Nicole Coufal, Sage Aronson, Kelsey Ladt, Catie Andersen, Mark Zeller, Stephen Rawlings, Denise Malicki, Gene W Yeo
Through the application of 3D printing and manual fabrication, we present an NP swab that can be created in a controlled environment.
COVID-19 screening using CDC and WHO primers
Catie Anderson, Mark Zeller
Protocol for COVID-19 screening using CDC and WHO primers.
