NextStrain builds we maintain to display our data in context of other publicly available data:
San Diego, California, Mexico, Mexico-California border region, New Orleans/Louisiana, New Mexico.
Overview
Consensus sequences
All consensus sequences will be deposited on GISAID and on NCBI under BioProject ID PRJNA612578. metadata.csv contains collection dates, location, originating labs and sequencing metrics: coverage and average depth.
| Location | Number of sequences |
|---|---|
| USA/California/San Diego | 843 |
| USA/Louisiana/New Orleans | 123 |
| USA/California/Imperial | 122 |
| MEX/BajaCalifornia/Tijuana | 39 |
| USA/California/Cruise_ship_2 | 32 |
| Asia / Jordan / Amman | 26 |
| USA/California/Cruise_ship_1 | 8 |
| Asia / Jordan / Irbid | 2 |
Raw read files
Raw read files for both Illumina and Oxford Nanopore data are available on,
- Google cloud.
- SRA under BioProject ID: PRJNA612578.
The sequencing is being performed using an amplicon-based sequencing scheme using PrimalSeq with artic nCoV-2019 scheme.
Nanopore data was processed using the artic-nCoV019 pipeline with minimap2 and medaka. Illumina data was processed using iVar (Grubaguh et al. Genome Biology 2019) with bwa.
Disclaimer. Please note that this data is still based on work in progress and should be considered preliminary. If you intend to include any of these data in publications, please let us know – otherwise please feel free to download and use without restrictions. We have shared this data with the hope that people will download and use it, as well as scrutinize it so we can improve our methods and analyses. Please contact us if you have any questions or comments – we’ll buy beers for #ResearchParasites that spot flaws and faults in the data and come up with improvements!
Andersen Lab
The Scripps Research Institute
La Jolla, CA, USA
[email protected]
Phylogeny
Data
Protocols
Collaborators
San Diego Partners
SEARCH Alliance, San Diego
San Diego County Public Health Laboratory
Tracy Basler, Jovan Shephard, Brett Austin, Raymond Chinn, Nikos Gurfield, Eric McDonald
Imperial County Public Health Department
Holly Maag, Stephen Munday, Paula Kriner
California Department of Public Health
Debra Wadford, Phacharee Arunleung, Kathleen Jacobson, Cindy Wong, Dominick Morales, John Bell, Sharon Messenger, Alice Chen
UCSD – Center for Advanced Laboratory Medicine
Jenny Shin, David Pride
UCSD – Division of Infectious Diseases and Global Public Health
Sara Browne
Rady Children Hospital, San Diego
Christina Clarke, Michelle Vanderpool, Teresa Mueller, Denise Malicki
Scripps Health, San Diego
Michael Quigley, Ellen Stefanski, Ian Mchardy
New Orleans
Allison Smither, Gilberto Sabino-Santos, Patricia Snarski, Lilia Melnik, Antoinette Bell, Kaylynn Genemaras, Arnaud Drouin, Dahlene Fusco, Robert Garry
Louisiana
Jeremy Kamil, Vaughn Cooper
Centro de Diagnóstico COVID-19, Tijuana, México
Idanya Rubí Serafín Higuera, Manuel Sanchez Alavez
Genomica Lab Molecular, México
Jonathan Gonzalez Garcia, Jose Roman Chavez Mendez, Jose Horacio Reyna Verdugo, Martin Gonzalez Ibarra, Luis Alberto Rangel Gonzalez
Infectolab, México
Samuel Navarro Alvarez, Carlos A. Cota Haros, Octavio Renteria Pacheco
Biolab, Jordan
Issa Abu-Dayyeh, Ahmad Tibi, Lama Hussein, Lina Mohammad, Zein Naber, Amid Abdelnour
Analysis Partners
Marc Suchard, UCLA
Lauren Gardner, Johns Hopkins
Philippe Lemey, KU Leuven
Alexander Watts & Kamran Khan, BlueDot
Emma Hodcroft, Nextstrain
Funding
