Protocol for SARS-CoV-2 sequencing with AVITI (Element Biosciences)
Allison Steedman, Alana Weiss, Christina Mac, Kelly Nguyen, Mark Zeller
This protocol is adapted from our PrimalSeq-LibAmp Illumina Sequencing protocol. The general approach is to amplify SARS-CoV-2 genomes in small (~250 bp) overlapping fragments using two separate, highly multiplexed PCR reactions.
High-throughput Wastewater SARS-CoV-2 Detection Pipeline
Caroline H Sheikhzadeh, Luis Gonzalez-barranca, Anna M Adams, Olivia Ott, Smruthi Karthikeyan, Lisa Marotz, Greg Humphrey
Streamlined, cost efficient process for sample collection, magnetic-bead based concentration, nucleic acid extraction, and multiplexed PCR for wastewater based detection of SARS-CoV-2 viral particles in up to 96 samples per run.
SARS-CoV-2 sequencing using PrimalSeq-LibAmp for ONT
Ezra Kurzban, Sarah Perkins, Catie Anderson, Madison Schwab, Mark Zeller
Protocol for sequencing SARS-CoV-2 using a long amplicon scheme for PrimalSeq. The average length of amplicons in this protocol is ~1200 nt, making this well-suited to sequencing on Oxford Nanopore platforms.
SARS-CoV-2 sequencing using ARTIC PrimalSeq-LibAmp
Catie Anderson, Mark Zeller
Updated protocol for sequencing SARS-CoV-2 using a shorter amplicon scheme for PrimalSeq. The average length of amplicons in this protocol is ~250 nt, making this well-suited to sequencing on NovaSeq, iSeq, and NextSeq platforms.
SARS-CoV-2 sequencing using ARTIC PrimalSeq-Nextera
Catie Anderson, Mark Zeller
Protocol for sequencing SARS-CoV-2 using ARTIC PrimalSeq. The average length of amplicons in this protocol is ~400 nt, making this well-suited to sequencing on the MiSeq.
3D printed nasopharyngeal swabs with wrapped rayon fibers
Justin Ryan, Nicole Coufal, Sage Aronson, Kelsey Ladt, Catie Andersen, Mark Zeller, Stephen Rawlings, Denise Malicki, Gene W Yeo
Through the application of 3D printing and manual fabrication, we present an NP swab that can be created in a controlled environment.
COVID-19 screening using CDC and WHO primers
Catie Anderson, Mark Zeller
Protocol for COVID-19 screening using CDC and WHO primers.
